Functional nanoprobes: design and use for sensing and imaging

Hedi Mattoussi and Mathias Brust - Nice, May 2011

Department of Chemistry / Bionano meeting, today 4pm, Brunner theatre

Hedi Mattoussi

Florida State University

Department of Chemistry and Biochemistry Tallahassee, FL 32306

Abstract

Inorganic nanocrystals (such as luminescent quantum dots, metallic and magnetic nanoparticles) exhibit unique optical and physical properties and they can be highly sensitive to potential interactions with proximal dyes and metal complexes.  We have developed approaches based on covalent and non-covalent self-assembly to conjugate a various biomolecules to CdSe-ZnS core-shell QDs rendered water-soluble using polyethylene glycol (PEG)-based modular multifunctional ligands.  In this presentation, we will focus on three aspects: 1) describe the ligand  design we have developed to promote the transfer of these materials to aqueous media and their conjugation to proteins and peptides; 2) the use of these materials and their bio-conjugates to image intracellular compartments and as sensing assemblies that are specific to target proteins and small molecules; and 3) explore the reverse process where the newly developed ligands are used to promote the synthesis of other nanocrystals such as gold nanoparticles.

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