PEGylate at your own risks…

paulPaul Free’s paper “PEGylation modulates the interfacial kinetics of proteases on peptide-capped gold nanoparticles” has been published today in the journal Chemical Communications. PEG, i.e. polyethylene glycol, is a polymer which is particularly good at preventing non specific interactions of proteins with surfaces. We have prepared nanoparticles which are decorated with a mixed layer containing both PEG and enzyme substrates.

In the paper, we present quantitative measurements of how the enzyme activity is modulated by the presence of the PEG. In short, the presence of the PEG strongly reduces the activity of the enzyme, presumably because it finds it hard to access its substrate.

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